Prof Dr. Joke A Bouwstra
Department
of Drug Delivery Technology
Leiden/Amsterdam Center for Drug Research
Einsteinweg 55
2300 RA, Leiden
The Netherlands
bouwstra@chem.leidenuniv.nl
Research
focus
-
Lipid organisation in stratum corneum: Biophysical methods (X-ray diffraction, electron diffraction, Infrared spectroscopy and confocal Raman spectroscopy) and electron microscopy are employed to provide information on the lipid organization and lipid composition in vitro and in vivo in humans
-
Design of novel formulations to repair the skin barrier
-
Microneedles and nanoparticles as delivery systems for drugs and vaccines
- Optimising culture
conditions of a reconstructed skin model
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The assessment of skin barrier properties in diseased skin, particular focusing on atopic eczema. In this topic we focus on the lipid composition using massaspectrometry and biophysical techniques. However, we are also able to measure the natural moisturizing factor and water levels.
Important publications
-
S.M. Bal, A.C. Kruithof, R. Zwier, E. Dietz, J.A. Bouwstra, J. Lademann and M.C. Meinke. Influence of microneedle shape on the transport of a fluorescent dye into human skin in vivo. J Contr Rel.147 (2010) 218-24
- V.S. Thakoersing,
M. Ponec and J.A. Bouwstra. Generation of human skin equivalents under
submerged conditions-mimicking the in utero environment. Tissue Eng
Part A. 16 (2010) 1433-41
- D. Groen. G.S.
Gooris and J.A. Bouwstra. New insights into the stratum corneum lipid
organization by x-ray diffraction analysis. Biophys J. 97 (2009) 2242-2249
- Z. Ding, E.
van Riet, S. Romeijn, G. Kersten, W. Jiskoot and J.A. Bouwstra. Immune
modulation by adjuvants combined with diphtheria toxoid administered
topically in mice after microneedle array pretreatment. Pharm Res
7 (2009) 1635-1643
- M. Janssens,
G.S. Gooris and J.A. Bouwstra. Infrared spectroscopy studies of mixtures
prepared with synthetic ceramides varying in head group architecture:
coexistence of liquid and crystalline phases. Biochim. Biophys. Acta
Biomembrames 1788 (2009) 732-742
- J. Caussin,
G.S. Gooris and J.A. Bouwstra. FTIR studies show lipophilic moisturizers
to interact with stratum corneum lipids, rendering the more densely
packed. Biochim Biophys Acta Biomembranes 1778 (2008) 1517-1524
- F.J. Verbaan,
S.M. Bal , D.J. van den Berg, J.A. Dijksman, M. van Hecke, H. Verpoorten,
A. van den Berg, R. Luttge and J.A. Bouwstra. Improved piercing of
microneedle arrays in dermatomed human skin by an impact insertion
method. J Control Rel 128 (2008) 80-88
- J.A. Bouwstra,
H.W.W. Groenink, J.A. Kempenaar, S. Romeijn and M. Ponec. The water
distribution and natural moisturizer factor in human skin equivalents
grown at various relative humidities. J Invest Dermatol 128 (2008)
378-388
- R. Rissmann,
H.W.W. Groenink, G.S. Gooris, M.H.M. Oudshoorn, W.E. Hennink, M. Ponec
and J.A. Bouwstra. Temperature-induced changes in structural and physicochemical
properties of vernix caseosa. J Invest Dermatol 128 (2008) 292-299
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G.S. Gooris and J.A. Bouwstra. Infrared spectroscopic study of stratum corneum model membranes prepared from human ceramides, cholesterol and fatty acids. Biophysical J., 92 (2007) 8: 2785-2795
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J.A. Bouwstra and M. Ponec. The skin barrier in healthy and diseased state. Biochim Biophys Acta 1758 (2006) 2080-2095